View enhanced pdf access article on wiley online library html view. Chrysin abrogates cisplatininduced oxidative stress, p53 expression, goblet cell disintegration and apoptotic responses in the jejunum of wistar rats volume 108 issue 9 rehan khan, abdul quaiyoom khan, wajhul qamar, abdul lateef, farrah ali, muneeb u. Nanoparticle formulations improve bioavailability and so may allow lowdose formulations of foodderived compounds such as curcumin to attenuate chronic systemic disease despite intrinsically low oral bioavailability. Wistar rat, a strain of albino laboratory rats developed at the institute. The establishment of metabolic syndrome model by induction of. Resveratrol reverses thioacetamideinduced renal assault. Pdf arsenicinduced oxidative stress, apoptosis and.
Pdf determination of median lethal dose of triazophos. The wistar rat, wistar hannover wistar han rat, and wistar unilever wu rat are outbred rat strains, while the wistar kyoto and wistar furth rats are. Handling and restraint of the rat a definition of both handling and restraint will serve to eliminate any confusion over these two terms in. The aim of this paper is to show various procedures useful to represent the results originating from the multivariate tpattern analysis of the behavioral response to pain in wistar rats tested in a hotplate and ip injected morphine or saline as a control. Lipid peroxidation and some antioxidant enzymes evaluation. The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and cpeptide in serum, between normal and diabetic rats. A tenth group of 10 rats received distilled water and served as control. Whilst there are reported cases of laboratory and animal technicians contracting disease from laboratory rats these are relatively rare and most of them occurred several years ago before the intro duction of the highquality diseasefree animals which are widely available for use today. Depending on the parameters of your experiments, cells from wistar rats, like wistar rat splenocytes, may offer experimental advantages. Health, general science and technology, general albinism psychological aspects antidepressants depression mood disorder depression, mental enzymes. Nigella sativa oil nso might have beneficial effects in the treatment of diabetic complications. Gross pathology in female wistar rats due to hexavalent mylaram jeevana latha, m lakshman, a anand kumar, a gopal reddy and d pramod kumar abstract an experiment was conducted for a period of four months by obtaining 120 female wistar rats to study the toxico pathological effects of.
Investigation on bioproperties and invivo antioxidant. The transport ratio of free amino acids from plasma to milk was not similar among the free amino acids tested. Laboratory bred rats were brought to the usa and established first at a chicago laboratory where they were used for neurological studies. Wistar rats encompass a number of rat substrains that have been derived. The wistar bloodline has contributed more strains of rats than any other line. Effect of coenzyme q10 on depression paradigms in male. For example, ingestion of free unbound glutamate may lead to. It is found combined with other elements, most commonly with.
Adult male wistar rats n 24, with an average weight of 230 g were. Origin derived at biological research laboratories limited brl, formerly rcc, now harlan laboratories ltd. Glial remodeling enhances shortterm memory performance in. Wistar rat article about wistar rat by the free dictionary. Brain sciences free fulltext different representation. The present investigation was carried out to determine the bioproperties and antioxidant potential of native carrageenan from kappaphycus alvarezii in comparison with commercial carrageenan under laboratory conditions in wistar albino rats. The animals were fasted for 12 h with free access to water before the experiment. The reduction in body weight gain of rats from groups exposed to 300 and mg kg. This study evaluated the phytochemicals, proximate and the toxicity effect of aqueous stem extract of senna alata using wistar rats. Hepatotoxicity and ultra structural changes in wistar rats treated with al2o3 nanomaterials s.
Testosterone levels of male wistar rats administered popular regular cola and sugarfree cola drinks for 3 and 6 weeks the effect of treatment with sugarfree cola brand and popular regular cola for 3 and 6 weeks on testosterone levels of male wistar rats are shown in table 3. Neuroprotective effect of egcg loaded nanoparticles. A total of 36 female wistar rats total 6 groups, n 6 per group 6 8 weeks old, weighing 150 gm were used in the study. Life span, morphology, and pathology of dietrestricted. The rats were randomly divided into four experimental groups. Free radical induced lipid peroxidation is believed to be one of the major causes of cell membrane damage leading to a number of pathological situations slater, 1984. Health status of male adult wistar rats from two experimental.
The development of todays laboratory rat has been essentially an american initiative and the great majority of the strains of these animals that are presently in use throughout the scientific world have originated in the usa. Periodontal disease induced in wistar rats experimental study. Free amino acid concentrations in plasma changed by both genetic background and lactation stage. Effect of melatonin on carbon tetrachloride induced. The rats were kept under standard laboratory housing conditions, with a 12 h light cycle 7 am to 7 pm, an ambient temperature of 22 c, with humidity between 40 and 60%, and free access to water and standard rat chow. The animals were given standard rat pellets and tap water ad libitum. In the present study, we tested whether this regulation is mediated by specific or all species of ffas. The wistar institute is a global leader in biomedical research with special expertise in cancer, immunology, infectious diseases and vaccine development. Toxicities caused by this compound in various tissues due to oxidative stress, increase of proinflammatory markers, and apoptosis have been reported.
Igs refers to animals bred using the charles river international genetic standardization igs program. Sipa1l1 is an early biomarker of liver fibrosis in ccl4. Rats included in this publication were housed singly or in pairs of the same sex in stainless steel wire mess cages with free access to tap water. Thus, this study was designed to unveil new noninvasive biomarkers of liver fibrosis in an in vivo model of fibrosiscirrhosis induction by ccl4 inhalation by using a labelfree.
Monosodium glutamate msg is a commonly used food additive and there is growing concern that excitotoxins such as msg play a critical role in the development of several hepatic disorders. P040803 evaluation of incision wound healing activity of scorzonera veratrifolia in wistar albino rats mert gecim, ayfer beceren, sezgin aydemir, semra sardas faculty of pharmacy, departments of pharmaceutical toxicology, marmara university, istanbul, turkey scorzonera veratrifolia s. Scientific poster historical background control data in the rcchan. Its longevity and high rate of spontaneous tumours make it an ideal. To develop a metabolic syndrome rat model by induction of fructose drinking water fdw in male wistar rats. Lobund wistar rat an overview sciencedirect topics. Maternal and fetal toxicity of wistar rats exposed to.
Wistar rat definition of wistar rat by the free dictionary. We previously showed that a fall in the plasma free fatty acid ffa level increases plasma corticosterone levels in rats by activating the hypothalamicpituitaryadrenal axis. Hematobiochemical changes of lead poisoning and amelioration with ocimum sanctum in wistar albino rats sujatha k. Determination of median lethal dose of triazophos with dmso in wistar rats article pdf available in asian journal of pharmaceutical and clinical research 74. Thioacetamide taa, a class 2btype carcinogen, is a potent toxicant. Thirtytwo adult male wistar rats, weighing 195205 g, were kept in metallic cages 4cage at laboratory animal house, faculty of veterinary medicine, suez canal university, egypt. They are also used in immunology as models for infectious diseases and autoimmune responses. Male wistar rats were treated orally with alcl3 at three doses. Most are derived from the albino wistar rat, which is still widely used. Transferred to charles river in 1997 and rederived into isolator maintained foundation colony. Biochemical and histopathological studies on female and male. This disambiguation page lists articles associated with the title wistar.
Toxicity evaluation of the aqueous stem extracts of senna. This study was done on 40 male wistar rats to evaluate the curative and protective effect of grape seeds extract on oxidative damage, inflammatory and apoptotic insult on lung tissue induced by cadmium chloride toxicity. Aluminium is a very reactive element and is never found as free metal in nature. Pdf mobile phone usage and male infertility in wistar rats. Rederived by glaxowellcome from han wistar stock supplied by brl. Group 1 was the control, while groups 2, 3 and 4 were each orally administered with a type of the energy drinks daily for 4 weeks. Researcheffect of venlafaxine on bone loss associated with. He later established wistar institute in philadelphia that will have a major role in the development of the rat as an important laboratory animal similar to the impact the jackson laboratory had on the development of the laboratory mouse. The dry powder of sgd was immersed in distilled water overnight. Effects of nori and wakameenriched meats with or without.
Charles river catalog laboratory animal resource center ucsf. They were kept under standard natural daylight rhythm with a temperature of 25c 1c and allowed to ad libitum diet and water supply. Wistar outbred rats show close genetic similarity with f344 inbred rats satoshi nakanishi1, tadao serikawa1, 2, and takashi kuramoto1 1institute of laboratory animals, graduate school of medicine, kyoto university, yoshidakonoecho, sakyoku. The animals were housed under standard laboratory conditions of light, temperature 212oc and relative humidity 555%. Wistar institute in philadelphia, giving rise to the ubiquitous presentday wistar strain of rats festing, 1979.
Effect of coenzyme q10 on depression paradigms in male wistar. A laboratory rat or lab rat is a rat of the species rattus norvegicus domestica which is bred and kept for scientific research. Group a was a dysmenorrhea model induced by estradiol benzoate injection combined with oxytocin. Chronic dosing with monosodium glutamate in albino wistar rats at a dose. Materials and methods experimental animals and diets twentyeight male wistar rats weighing. After two and four weeks of treatment, five animals from each group were killed. Pdf on mar 1, 1998, b t clause and others published the wistar institute archives.
Thirty adult wistar rats weighing between 150 g and 200 g were used for this study. The physiological changes with regard to food and fluid intake, as well as calorie intake, were measured. At present, several procedures are used for staging liver fibrosis. Eighteen male wistar rats were fed with fdw 20% and fdw 25% for a duration of eight weeks. Wistar outbred rats show close genetic similarity with. The aim of this study was to evaluate the effects of a diet containing gm soybean meal rur on male and female wistar rats. Nutrients free fulltext lowdose curcumin nanoparticles. Black seed thymoquinone improved insulin secretion. Group 1 was the control, while groups 2, 3 and 4 were each orally administered with a type of the. Different forms of aluminum are environmental xenobiotics that induce free radicalmediated cytotoxicity and reproductive toxicity. Molecules free fulltext effects of chronic administration of.
Depending on the parameters of your experiments, cells from wistar rats like wistar rat pbmcs may offer experimental advantages. Beneficial anthropometric and lipoglycemic impacts of. The wistar rat is an outbred stock, used in all fields of medical and biological research. We present an in vivo kinetic study of nisrelated hepatic iodine uptake in an aggressive model of hepatocarcinoma induced by. Hepatotoxicity and ultra structural changes in wistar rats. Wistar rat synonyms, wistar rat pronunciation, wistar rat translation, english dictionary definition of wistar rat. Sixty weaned wistar albino rats 30 females and 30 males were randomly assigned to three groups of ten rats for each group per each sex. A significant decrease in protein kinase c and total sperm count along with increased apoptosis were observed in male wistar rats exposed to mobile phone frequencies 2 hday x 35 days at 0. Five rats from each group were sacrificed every 4 months for 24 months for histological and biochemical monitoring.
A study was conducted to compare health status of male adult wistar rats from two experimental animal houses of ufmg with literature data of spf free from. Rat pbmcs can be used for a wide variety of immunologybased applications. To evaluate the effect of aead in ccl4 induced testicular toxicity, 20 adult male wistar rats were equally divided into 4. Wist rat in chronic toxicity studies scientific poster female wistar han outbred rats as a model of obesity when fed high fat diets.
While less commonly used for research than mice, rats have served as an important animal model for research in psychology and biomedical science. Diabetes mellitus is one of the metabolic diseases having several complications. In the present study, the ameliorative effect of the aqueous leaf extract of adansonia digitata aead was evaluated in carbon tetrachloride ccl4 induced testicular toxicity in wistar rats. Wistar rat medical definition merriamwebster medical. In acute toxic study, no toxicity was observed in rats at the dose of 2000 mgkg of both carrageenans, hence three different doses 500, 750 and mg. The albino lobundwistar lw rat is used as a model of prostate cancer and was first described by pollard 1973 in a colony of germfree outbred wistar rats. A laboratory rat or lab rat is a rat of the species rattus norvegicus domestica which is bred and.
Wistar rat splenocytes can be used for a wide variety of immunologybased applications. Protective effect of ethanolic extract of tabernaemontana. Report by national journal of physiology, pharmacy and pharmacology. Effect of melatonin on carbon tetrachloride induced kidney. The laboratory rat handbook of experimental animals. They were purchased, housed and fed under laboratory conditions and had free access to feed and water, and they were acclimatized to laboratory conditions for 2 weeks before commencement of the experiment. The aim of this study was to investigate the development of collageninduced arthritis cia in male and female wistar and lewis rats and establish relationships between clinical status and the. All rats were given free access to a normal chow diet and water. Gln reduced wc and serum lipopolysaccharide lps in overweight volunteers. The aim of the present study was to analyze the effects of physical training in different intensities on the aerobic capacity and lactate production by the isolated soleus muscle of wistar rats. These mice are free of all of the agents listed above in the vafplus mouse. The widespread use of the wistar rats, however, is a function of the ingenuity of milton greenman who.
Sixty wistar albino rats were fed a chow diet and 60 the same diet supplemented initially with 2% carbonyl iron for 12 months, followed by 0. Mccormick, in a comprehensive guide to toxicology in nonclinical drug development second edition, 2017. The effect of germfree life and dietary restriction dr on life span and pathology was investigated in isolator housed germfree gf and conventional cv lobundwistar rats fed either ad libitum or restricted to 12 grams per day 70 % of adult ad libitum intake of a. Differences in free amino acid concentrations in milk between. Wild rats act as a reservoir for several zoonotic agents. To investigate molecular mechanisms, gln or ala was given to wistar rats on a highfat diet hfd, and metabolic parameters, euglycemic hyperinsulinemic clamp with tracers, and western blot were done. The effect of germ free life and dietary restriction dr on life span and pathology was investigated in isolator housed germ free gf and conventional cv lobund wistar rats fed either ad libitum or restricted to 12 grams per day 70 % of adult ad libitum intake of a natural ingredient diet from weaning. Specific sample sizes are indicated in the figure legends. Black seed thymoquinone improved insulin secretion, hepatic. Effects of nori and wakameenriched meats with or without supplementary cholesterol on arylesterase activity, lipaemia and lipoproteinaemia in growing wistar rats volume 106 issue 10 raul oliverodavid, adriana schultzmoreira, miguel vazquezvelasco, laura gonzaleztorres, sara bastida, juana benedi, maria isabel sanchezreus, maria jose gonzalezmunoz, francisco j. Pdf in summary, the creation and maintenance of the wistar rats as standardized. Morphologic features and incidence of spontaneous hyperplastic and neoplastic mammary gland lesions in wistar rats nabila j. Thirtytwo mature male wistar rats were equally divided into four experimental groups. Wistar rats are more active than other strains such as sprague dawley rats.
The common domestic rat, which is found worldwide and is a destructive pest of crops and stored food and a carrier of disease. Using rats as a research model to investigate the effect of human. Wistar rats definition of wistar rats by medical dictionary. This study aimed to evaluate the chronic effect of capsaicin administration on biomarkers of kidney injury in an experimental rat model of diabetes. Evaluation of incision wound healing activity of scorzonera. We studied the aluminum trichloride alcl3 toxicity on adult rat testes. In 1906, some of this stock was transferred to the wistar institute in philadelphia, giving rise to the ubiquitous presentday wistar strain of rats festing, 1979. In acute toxicity test, aqueous dried stem extract of senna alata were administered orally up to 10 gkg body weight to male wistar rats. Please tell us where you read or heard it including the quote, if possible. Chrysin abrogates cisplatininduced oxidative stress, p53. But, the precise correlation between age of laboratory rats and human is still a subject of debate. Effect of coenzyme q10 on depression paradigms in male wistar rats and swiss albino mice. Gross pathology in female wistar rats due to hexavalent. To achieve this objective, al2o3 of three different sizes 30nm, 40nm and bulk was orally administered for 28 days to 9 groups of 10 wistar rats each, at the dose of 500, and 2000 mgkgrat.
Regulation of hypothalamicpituitaryadrenal axis by. He later established wistar institute in philadelphia that will have a major role in the development of the rat as an important. Life span, morphology, and pathology of dietrestricted germ. Histological studies of the effects of monosodium glutamate. However, it seems to be precluded by the rapid efflux of taken up iodine from most transduced xenografted tumors. Jul 18, 2019 roundup ready became the most cultivated genetically modified gm crop in the world. Adult female wistar rats n24 weighing between 182 to 186grams were randomly assigned into three groups a, b and c of n8 in each group. Laboratory rats introduction origin and development. Impact of prolonged intake of sugarfree carbonated soft. However, these methods may involve clinical complications andor present diagnostic uncertainty mainly in the early stages of the disease. Longterm radioiodine retention and regression of liver. The wistar rat, wistar hannover wistar han rat, and wistar unilever wu rat are outbred rat strains, while the wistar kyoto and wistar furth rats are inbred strains. Chronic toxicity of low dose monosodium glutamate in albino wistar. The zucker fatty rat as a genetic model of obesity and hypertension pdf.
Wistar rat definition of wistar rat by medical dictionary. Material and methods ten male adult wistar rats obtained from laboratory animal facility centre for experimental medicine, iuliu hatieganu university of medicine and pharmacy, with average weight of 180200 g, were included in this study. The histochemical effect of monosodium glutamate was investigated on the liver of adult wistar rats. Collageninduced arthritis and proantioxidant status in. Resveratrol rsv, on the other hand, has demonstrated antioxidant and antiinflammatory effects in different. The experimental protocol for surgical procedures and animal treatments was duly. The body weights, food consumption, hematology, serum biochemistry, organ weight, and histopathological examination were compared between rats fed on gm soybean and those fed nongm. Origin and development the wild brown rat rattus norvegicus is thought to have had its original habitat in the temperate areas of what is now the ussr, from the caspian sea to northern china. In summary, the creation and maintenance of the wistar rats as standardized animals can be attributed to the breeding work of helen dean king, coupled with the management and husbandry methods of milton greenman and louise duhring, and with supporting documentation provided by henry donaldson.
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